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HBIO 320 - Muscle Physiology | Midterm 2, Exams of Human Physiology

HBIO 320 - Muscle Physiology | Midterm 2

Typology: Exams

2024/2025

Available from 03/13/2025

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HBIO 320 - Muscle Physiology | Midterm 2
A contraction that creates force without moving a load is an ________ contraction. ✔✔Isometric
The ______ ____ stretch and absorb the force produced by contracting sarcomeres. ✔✔Elastic Elements
TIme to Peak Tension, Relaxation Times (50 and 90%) are all determined by the duration of the
_______ transient. ✔✔Calcium
Rate of Tensin development is ________ of the calcium transient. ✔✔Independent
Time lag from ____ release at the NMJ to ______ _____ is limited by diffusion across synapse.
✔✔ACh, Muscle Depolarization
A single neural stimulation produces a single contraction or _______. ✔✔Twitch (7-100 ms)
What are the three phases of a twitch? ✔✔Latent Period, Contraction Phase, Relaxation Phase
How long is the latent period? ✔✔~1-2ms
How long is the contraction phase? ✔✔2s - 10s of msec
How long is the relaxation phase? ✔✔(~25 msec)
This period is the delay before Calcium release as action potential moves through sarcolemma.
✔✔Latent Period
This period has calcium ions binding to troponin and tension builds to peak. ✔✔Contraction Phase
This period has the SERCA causing the sarcoplasmic calcium levels to fall, active sties are covered;
tension decreases ✔✔Relaxation Phase
Sustained muscular contractions require ______ stimuli. ✔✔Repeated
A single ____ will not produce normal movement. (requires many cumulative twitches). ✔✔Twitch
Stimuli arrive at a frequency that prevents complete relaxation (SERCA cannot reabsorption all Ca2+)
so that tension builds upon the previous twitch ✔✔Complete or Unfused Tetanus
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HBIO 320 - Muscle Physiology | Midterm 2

A contraction that creates force without moving a load is an ________ contraction. ✔✔Isometric The ______ ____ stretch and absorb the force produced by contracting sarcomeres. ✔✔Elastic Elements TIme to Peak Tension, Relaxation Times (50 and 90%) are all determined by the duration of the _______ transient. ✔✔Calcium Rate of Tensin development is ________ of the calcium transient. ✔✔Independent Time lag from ____ release at the NMJ to ______ _____ is limited by diffusion across synapse. ✔✔ACh, Muscle Depolarization A single neural stimulation produces a single contraction or _______. ✔✔Twitch (7-100 ms) What are the three phases of a twitch? ✔✔Latent Period, Contraction Phase, Relaxation Phase How long is the latent period? ✔✔~1-2ms How long is the contraction phase? ✔✔2s - 10s of msec How long is the relaxation phase? ✔✔(~25 msec) This period is the delay before Calcium release as action potential moves through sarcolemma. ✔✔Latent Period This period has calcium ions binding to troponin and tension builds to peak. ✔✔Contraction Phase This period has the SERCA causing the sarcoplasmic calcium levels to fall, active sties are covered; tension decreases ✔✔Relaxation Phase Sustained muscular contractions require ______ stimuli. ✔✔Repeated A single ____ will not produce normal movement. (requires many cumulative twitches). ✔✔Twitch Stimuli arrive at a frequency that prevents complete relaxation (SERCA cannot reabsorption all Ca2+) so that tension builds upon the previous twitch ✔✔Complete or Unfused Tetanus

Explain how an intact and mechanically skinned muscle fiber. ✔✔The sarcolemma is simply rolled back with forceps forming a cuff and the transverse t-tubules seals off to form a closed compartment after skinning. Explain what chemical skinning or permeabilizing does. ✔✔Involves the use of a chemical reagent to permeabilize the various membranes of the fiber _______ or -_______ are thought to act primarily on the surface of membranes (sarcolemma) and t- tubules by binding cholesterol, which is largely absent from the SR. (Selective) ✔✔Saponin or Beta- Aescin _____ and ______- _____ destroy all the membrane structures and leave only the contractile machinery intact. (nonselective) ✔✔Glycerol and Triton-X 100 What are the tension parameters for a muscle twitch? ✔✔1. Peak Twitch Tension

  1. Time to Peak Twitch Tension
  2. Rate of Tension Development
  3. 1/2 Relaxation Time
  4. 90% Relaxation Time ______, _____, and _____ are all determined by the duration of the calcium transient. ✔✔TPT (time to peak twitch tension), RT 50% (1/2 relaxation time, RT 90% (90% Relaxation Time) Rate of Tension Development is _______ of the ______ transient. Instead it depends on the rate of formation of tightly bound _____-_______. ✔✔Independent, Calcium, cross-bridges Peak Twitch tension is the function of the _______ and ______. ✔✔TPT (time to peak twitch tension) and dP/dT (rate of tension developed) Time from arrival of AP to contraction determined by activation of _____ and exposure of ___-_____ ____. ✔✔DHPR, G-Actin Sites Duration of _____ from a single AP is much longer than AP duration. What is it? ✔✔(7.5 - 80 ms) Single twitches _____ _____ in time lead to small amounts of tension generated. ✔✔Widely Separated Increasing tension results from _____ of twitches. ✔✔Summation Summation occurs when the next twitch arrives while some _______-_____ are still intact and some _____ remains in the sarcoplasm--resulting in increasing tension. ✔✔Cross-Bridges; Calcium

A ______-_______ ______ is an active stretch (eccentric contraction) of a muscle followed by an immediate shortening (concentric contraction) of that same muscle. ✔✔Stretch-Shortening Cycle (SSC) More force by the SSC can be caused by... (4) ✔✔(1) Ca2+-dependent increase in titin stiffness (2) the development of half sarcomere nonuniformities (3) a stretch-induced increase in the number of attached cross bridges (4) an increase in the average cross-bridge force. Calcium binding to titin can ________ its _______. ✔✔Increase, Stiffness Titin binding to actin can ____ the ____ ______. ✔✔Reduce, Spring Length The primary contributor to passive tension is the mega-protein _____. ✔✔Titin Calcium appears to initially stiffen titin by binding to ______ in the ______ region. ✔✔Glutamates, PEVK What are the four functions of Titin? ✔✔1. Centering of A bands

  1. Strain-Sensing
  2. Elastic Recoil and Refolding of Titin domains as an energy source
  3. Stiffness Alterations This term is caused by a strain being sensed by a thick filament protein and being transmitted via MyBPC to the thin filament. ✔✔Strain-Sensing Increase calcium sensitivity during stretch causes a.... ✔✔Left Hand Shift and Increase in Force The results indicate that _______ and ________ activated _______ activities of myosins were proportional to the speed of shortening of their respective muscles. ✔✔Actin, Calcium, ATPase Peak power is obtained at _____ loads and _____ velocities. ✔✔Intermediate, Intermediate Power is ______ times ______. ✔✔Force, Velocity ________ contractions have positive force/tension, but negative velocity. ✔✔Eccentric _____ contractions are known to lengthen the sarcomere. ✔✔Eccentric _____ contractions are known to shorten the sarcomere. ✔✔Concentric

Sarcomeres in series are known to have maximum contraction ____ that is ______ of fiber length. ✔✔Force, Independent Sarcomeres in series are known to have contraction velocity that is directly _______ to the _____ _____. ✔✔Proportional; Fiber Length Muscle fibers in parallel have maximum contraction ____ that is directly ______ on the ____-______ _____. ✔✔Force, Dependent, Cross-Sectional Area Contraction _____ is independent of cross-sectional area. ✔✔Velocity Power stroke of the myosin produces ____-____ piconewtons and travels ___ nm. ✔✔ 3 - 4 pN, 10nM Eccentric contractions can cause a _____ ____ which means cross-bridges may be "forcibly removed." ✔✔Break Point Color from muscle fiber comes from ____ and _____. ✔✔Myoglobin and Mitochondria What are the histochemical types of Myosin ATPase activity? (3) ✔✔Type I, Type IIA, Type IIB What are the types of immunohistochemical identifications with Myosin Heavy Chain Antibodies? (4) ✔✔Type 1, Type 2A, Type 2X, Type 2B ____ fibers are rich in SDH, which have _____ oxidative capacity. ✔✔Dark, High _____ fibers have low SDH content, which have ______ oxidative capacity. ✔✔Light, Low The marker enzyme used for histochemical labeling of muscle fiber types is _______ _____. ✔✔Succinate Dehydrogenase _____ pH inhibits slow ATPases. ✔✔High _____ pH inhibits fast ATPases. ✔✔Low In human muscle, type _____ can be identified by reacting the section with BAD5 and SC71. ✔✔Type IIx Immunochemical identification of muscle fiber types use _______. ✔✔Antibodies Type I Fibers always contain ______ and ______ and _______. ✔✔MYH6, MYH7, and MYL