



Study with the several resources on Docsity
Earn points by helping other students or get them with a premium plan
Prepare for your exams
Study with the several resources on Docsity
Earn points to download
Earn points by helping other students or get them with a premium plan
Community
Ask the community for help and clear up your study doubts
Discover the best universities in your country according to Docsity users
Free resources
Download our free guides on studying techniques, anxiety management strategies, and thesis advice from Docsity tutors
HBIO 320 - Muscle Physiology | Midterm 2
Typology: Exams
1 / 6
This page cannot be seen from the preview
Don't miss anything!
A contraction that creates force without moving a load is an ________ contraction. ✔✔Isometric The ______ ____ stretch and absorb the force produced by contracting sarcomeres. ✔✔Elastic Elements TIme to Peak Tension, Relaxation Times (50 and 90%) are all determined by the duration of the _______ transient. ✔✔Calcium Rate of Tensin development is ________ of the calcium transient. ✔✔Independent Time lag from ____ release at the NMJ to ______ _____ is limited by diffusion across synapse. ✔✔ACh, Muscle Depolarization A single neural stimulation produces a single contraction or _______. ✔✔Twitch (7-100 ms) What are the three phases of a twitch? ✔✔Latent Period, Contraction Phase, Relaxation Phase How long is the latent period? ✔✔~1-2ms How long is the contraction phase? ✔✔2s - 10s of msec How long is the relaxation phase? ✔✔(~25 msec) This period is the delay before Calcium release as action potential moves through sarcolemma. ✔✔Latent Period This period has calcium ions binding to troponin and tension builds to peak. ✔✔Contraction Phase This period has the SERCA causing the sarcoplasmic calcium levels to fall, active sties are covered; tension decreases ✔✔Relaxation Phase Sustained muscular contractions require ______ stimuli. ✔✔Repeated A single ____ will not produce normal movement. (requires many cumulative twitches). ✔✔Twitch Stimuli arrive at a frequency that prevents complete relaxation (SERCA cannot reabsorption all Ca2+) so that tension builds upon the previous twitch ✔✔Complete or Unfused Tetanus
Explain how an intact and mechanically skinned muscle fiber. ✔✔The sarcolemma is simply rolled back with forceps forming a cuff and the transverse t-tubules seals off to form a closed compartment after skinning. Explain what chemical skinning or permeabilizing does. ✔✔Involves the use of a chemical reagent to permeabilize the various membranes of the fiber _______ or -_______ are thought to act primarily on the surface of membranes (sarcolemma) and t- tubules by binding cholesterol, which is largely absent from the SR. (Selective) ✔✔Saponin or Beta- Aescin _____ and ______- _____ destroy all the membrane structures and leave only the contractile machinery intact. (nonselective) ✔✔Glycerol and Triton-X 100 What are the tension parameters for a muscle twitch? ✔✔1. Peak Twitch Tension
A ______-_______ ______ is an active stretch (eccentric contraction) of a muscle followed by an immediate shortening (concentric contraction) of that same muscle. ✔✔Stretch-Shortening Cycle (SSC) More force by the SSC can be caused by... (4) ✔✔(1) Ca2+-dependent increase in titin stiffness (2) the development of half sarcomere nonuniformities (3) a stretch-induced increase in the number of attached cross bridges (4) an increase in the average cross-bridge force. Calcium binding to titin can ________ its _______. ✔✔Increase, Stiffness Titin binding to actin can ____ the ____ ______. ✔✔Reduce, Spring Length The primary contributor to passive tension is the mega-protein _____. ✔✔Titin Calcium appears to initially stiffen titin by binding to ______ in the ______ region. ✔✔Glutamates, PEVK What are the four functions of Titin? ✔✔1. Centering of A bands
Sarcomeres in series are known to have maximum contraction ____ that is ______ of fiber length. ✔✔Force, Independent Sarcomeres in series are known to have contraction velocity that is directly _______ to the _____ _____. ✔✔Proportional; Fiber Length Muscle fibers in parallel have maximum contraction ____ that is directly ______ on the ____-______ _____. ✔✔Force, Dependent, Cross-Sectional Area Contraction _____ is independent of cross-sectional area. ✔✔Velocity Power stroke of the myosin produces ____-____ piconewtons and travels ___ nm. ✔✔ 3 - 4 pN, 10nM Eccentric contractions can cause a _____ ____ which means cross-bridges may be "forcibly removed." ✔✔Break Point Color from muscle fiber comes from ____ and _____. ✔✔Myoglobin and Mitochondria What are the histochemical types of Myosin ATPase activity? (3) ✔✔Type I, Type IIA, Type IIB What are the types of immunohistochemical identifications with Myosin Heavy Chain Antibodies? (4) ✔✔Type 1, Type 2A, Type 2X, Type 2B ____ fibers are rich in SDH, which have _____ oxidative capacity. ✔✔Dark, High _____ fibers have low SDH content, which have ______ oxidative capacity. ✔✔Light, Low The marker enzyme used for histochemical labeling of muscle fiber types is _______ _____. ✔✔Succinate Dehydrogenase _____ pH inhibits slow ATPases. ✔✔High _____ pH inhibits fast ATPases. ✔✔Low In human muscle, type _____ can be identified by reacting the section with BAD5 and SC71. ✔✔Type IIx Immunochemical identification of muscle fiber types use _______. ✔✔Antibodies Type I Fibers always contain ______ and ______ and _______. ✔✔MYH6, MYH7, and MYL